Porphyromonas gingivalis  is a keystone pathogen in periodontitis, known for its ability to invade gingival epithelial cells and persist intracellularly. Conventional antimicrobials are often ineffective against intracellular pathogens, and natural products remain poorly explored in this context. Here, we investigated the antimicrobial effects of  Boswellia serrata  extract and its bioactive compounds on the dynamics of  P. gingivalis  infection   in human gingival epithelial

Highlights Senescent cells drive aging, chronic disease, and tissue dysfunction Senotherapeutics span senolytics, senomorphics, immunotherapy, and restoration Target selection, screening readouts, and delivery platforms are linked to senescent-state biology to improve selectivity and translation Abstract Cellular senescence is a stress-induced cellular state that contributes to tissue dysfunction, chronic inflammation, and a broad range of aging-associated pathologies. The ac

Highlights Cancer cells hijack mitochondria from many immune cells Mitochondria loss by immune cells impairs innate and adaptive anti-tumor immunity Fusion of hijacked and endogenous cancer cell mitochondria triggers cGAS STING activation cGAS-STING activation promotes lymph node metastasis through type I interferon signaling Summary Although the immune system is a significant barrier to tumor growth and spread, established tumors evade immune attack and frequently colonize i

Highlight T6SS effector TseVF demonstrates apparent bacterial killing activity in the periplasm. TseVF interacts with phosphate lipid molecules and compromises the integrity of the cell membrane. The C-terminal region of TseVF is responsible for its bactericidal activity. 4. TsiVF binds to and neutralizes TseVF toxicity. TseVF secretion and bactericidality requires the C terminus of a cognate VgrG protein (TssI2_b). Abstract Vibrio fluvialis  ( V. fluvialis ) is a halophilic

Highlights A total of 833 glacier-derived potential pathogens were identified across habitats. Altitude and TOC drive distinct patterns of pathogen diversity in glacier habitats. Moraine and cryoconite are key sources of downstream pathogen dispersal. Community assembly is mainly stochastic, with network structure shaping selection. Cryospheric biosafety risks emerge via airborne and meltwater transmission pathways. Abstract Glacier retreat driven by global warming is releasi

Parabacteroides  is a  Gram-negative ,  anaerobic , non- spore -forming genus from the family  Tannerellaceae . First isolated from fecal specimen in 1933, type strain  Parabacteroides distasonis  was originally classified under the name  Bacteroides distasonis.  The strain was re-classified to form the new genus  Parabacteroides  in 2006.  Parabacteroides  currently comprise 21 phylogenetically, ecologically, and metabolically diverse species, 11 of which are listed in the t

Highlights Bacillus sp. HC-9 controls honeysuckle leaf spot caused by A. alternata. Strain HC-9 inhibits eight phytopathogenic fungi with strong antifungal activity. Strain HC-9 induces ROS and activates defense enzymes in honeysuckle leaves. Field trials show HC-9 achieves up to 80.52% disease control efficiency. Abstract Honeysuckle leaf spot disease, caused by Alternaria alternata , severely affects the yield and quality of honeysuckle crops. Biological control using nati

Highlights Identification of the TamA protein as a highly conserved antigen across diverse Klebsiella pneumoniae strains, making it an ideal target for a broad-spectrum subunit vaccine. Development and evaluation of a recombinant TamA protein vaccine that significantly enhances immune protection against a highly virulent Klebsiella pneumoniae infection in a murine model. Demonstration of TamA-induced specific antibodies that effectively inhibit bacterial adherence to epithe

Chlamydiosen  sind durch verschiedene  Chlamydien  (bestimmte  Bakterienarten ) verursachte  Infektionskrankheiten . Sie betreffen das  Auge  (als  Trachom ), die  Atemwege  und die  Lunge  (als  Pneumonie ) und den  Urogenitaltrakt  (Harn- und Geschlechtsorgane) als  sexuell übertragene Krankheit . Von  genitalen  Chlamydien-Infektionen sind überwiegend Jugendliche und junge Erwachsene im Alter von 15 bis 24 Jahren betroffen. Urogenitale Chlamydien-Infektionen verlaufen häuf

Highlights Phospholipid diversity shapes membrane properties for growth and stress adaptation Bacteria remodel phospholipid head groups and acyl chains to resist antimicrobials Phospholipid and its metabolism serve as promising potential antibacterial targets Abstract Bacterial infections have posed a serious threat globally. The discovery of new targets and the development of novel antimicrobial agents are urgently needed to combat these bacterial infections. The bacterial m

Highlights First systematic report of Niallia taxi BN5 with plant growth-promoting (PGP) activity, filling the Niallia PGP mechanism gap (previously studied for bioremediation/antibacterial functions). Multi-omics (genome/transcriptome/qPCR) show BN5 has a complete trp operon for constitutive IAA biosynthesis (17.64 μg/mL); tryptophan regulates trp/motility genes. BN5 enhances cucumber growth (height +24.29%, stem +11.23%), yield (+17.55%) and quality (vitamin C +17.2%, sol

Many shots seem to have “off-target” benefits, such as lowering the risk of dementia, studies have found. Let’s be clear. The primary reason to be vaccinated against shingles is that two shots provide 90 percent protection against a painful, blistering disease that a third of Americans will suffer in their lifetimes, one that can cause lingering nerve pain and other nasty long-term consequences. The most important reason for older adults to be vaccinated against the respirat

Highlights Distinct microbial communities are found in peritoneal dialysis (PD) and paired hemodialysis (HD) patients, despite similar microbiome diversity. Serum p-cresyl sulfate (PCS) levels are significantly lower in PD patients compared to paired HD patients. Altered microbial composition in PD patients, featuring more opportunistic pathogens and fewer beneficial bacteria, is linked to differences in metabolite profiles. Fecal transplant experiments confirm that the HD-as

Highlights Salinity and nutrient availability affect the utilization and secretion of amino acids by deep biosphere microbial consortia Anoxic deep biosphere enrichment cultures were compared to Desulfovibrio desulfuricans Amino acids and organic acids were degraded under different nutrient conditions Phe and Val degradation remained unaffected by changes in nutrient availability Metabolic pathways involving Phe, Cys and Met were most prominent Abstract The deep terrestrial s

Highlights Mixed reed and willow vegetation boosts denitrification more than monocultures. nirK  denitrifiers are major drivers of N removal variation over  nirS . Soil depth reshapes denitrifiers:  nirS  depth-sensitive,  nirK  nutrient-responsive. Reed-willow boosts network complexity with depth to foster microbial interactions. Bradyrhizobium, Sinorhizobiu m, and  Mesorhizobium  are key to mitigating N loss. Abstract Estuarine wetlands are critical biogeochemical hotspots

Highlights Pseudomonas, Escherichia and Prevotella dominated DFU assemblage across visits Alcaligenes dominated healed DFU while Enterococcus was abundant in worsened DFU High HbA1c favored Pseudomonas while Prevotella was abundant in lower HbA1c samples Amputated DFUs had increased abundance of Escherichia and reduced Staphylococcus Microbiome functional profiles in worsened DFU were enriched in virulence factors Abstract Microbial communities in infected diabetic foot ulcer

Highlights Butyrolactol A synergizes with caspofungin against cryptococci and Candida auris BLA restores echinocandin efficacy by targeting the Apt1-Cdc50 flippase complex Cryo-EM shows BLA traps the Apt1-Cdc50 phospholipid flippase in a dead-end state BLA blocks lipid transport, disrupts membrane homeostasis, and boosts drug uptake Summary Fungal infections cause millions of deaths annually and are challenging to treat due to limited therapeutic options and rising resistance

Highlights Viruses are largely overlooked in outdoor aeromicrobiological studies Based on current knowledge in aeromicrobiology, a total of 10 21 virus particles are estimated to occupy clouds globally Although very dilute, these may contribute to the atmospheric life cycle of microorganisms Virus-bacteria interactions in clouds would have many implications in ecology and the Earth’s microbiome Abstract Clouds are aqueous atmospheric systems hosting diverse and active microo

Highlights • V olume electron microscopy unveils intensive membrane networks in insect symbiotic cells • These tubenets are formed by intracellular bacteria, the insect’s nutritional symbionts • In situ high spatial resolution chemical analysis shows tubenets’ enrichment in sugar • Bacterial tubenets increase the interface of exchange, maximizing nutrient acquisition Summary Symbiosis is widespread in nature and plays a fundamental role in organism adaptation and evolution.

Highlights The Borrelia restriction-modification system (RMS) differs by species group. The RMS affects the transformation efficiency of Borrelia. Increase in transformation efficiency observed with an in vitro methylated vector. Transformation efficiency increased in low-passage Borrelia strains. In vitro methylation can be a facilitating tool for Borrelia genetic manipulation. Abstract Borrelia, a highly prevalent tick-borne pathogen, has a genome with a linear chromoso