Highlights MT is dominated by Saccharomyces and Lactobacillus. MT has high ester, JS features elevated aldehydes, reflecting regional sensory. Saccharomyces and Pichia correlate with esters. Abstract This research focused on Maotai-flavour Baijiu from three representative production regions in the Chishui River Basin, namely Maotai Town (MT), Jinsha (JS), and Renhuai (RH). By integrating metabolomics and macrogenomics techniques, the study analyzed the differences in volatile

Highlights A significant proportion (∼11%) of  bla KPC genes were chromosome-integrated in  P. aeruginosa . Tn 4401 /Tn 7247 -like structures were the major transposons mobilizing  bla KPC. Tn 4401 - and Tn 7247 -like structures differed in geographic distribution and plasmid/chromosome localization. IS 26  and IS 6100  formed prevalent chimeric structures with truncated transposons to mobilize  bla KPC. Integrative mobilizable elements (IMEs) facilitated inter-cellular trans

Highlights Impairment of circadian rhythm by long-term rest-phase time-restricted feeding (TRF) induces bone loss and gut microbiota dysbiosis in male mice TRF feeding mice showed a decreased  Muribaculaceae  and propionate in feces Muribaculaceae  abundance level is positively correlated with bone mass Fecal microbiota transplantation from TRF donors to germ-free recipients leaded to increased Th17 cell populations Increased Th17 cell populations enhanced osteomorphs fusion

Highlights Aggregatibacter actinomycetemcomitans  is a causative agent of periodontitis. Fimbriae are key modulators of  A. actinomycetemcomitans  virulence. Fimbriae drive aggregation, colonization, immune evasion and pathogenicity. Fimbriae limit bacterial vesicle and extracellular cytoplasmic protein release. Fimbriae are key therapeutic targets to control periodontal disease. Abstract Aggregatibacter actinomycetemcomitans  is a major contributor to aggressive periodontiti

Highlights Glutathione biosynthesis is required for GSH production in  A. baumannii Loss of GSH impairs stress resistance and motility gshA, gshB , and  gsnoR  mutants are sensitive to nitrosative stress and impaired in biofilm formation GSH deficiency alters expression of pili, siderophore, and metabolic genes Exogenous GSH or GSH-containing media restores biofilm formation in mutants Abstract Acinetobacter baumannii  is a Gram-negative nosocomial bacterium that is a member

Background Quantifying human health impacts throughout plastics lifecycles can inform global action against pollution that promotes sustainability across environmental, economic, and health concerns. Methods We combined material flow analysis, using the Plastics-to-Ocean model, with lifecycle assessment (LCA) to quantitatively compare disability-adjusted life-years (DALYs) associated with plastics lifecycles, under different global consumption and waste management scenarios b

Highlights Extreme temperatures caused dysbiosis in lung and gut of mice. Low temperature affects pentanoic and butyric acid production in mice. High temperature promotes intestinal inflammation in mice. Extreme temperatures altered the proteome profile and pathways in the lung and gut of mice. Abstract Climate change has a strong effect on respiratory health, the effects of extreme temperatures on the lung-gut axis remains unclear. This study investigates the impact of extre

Highlights H5-naive individuals show low but detectable A/Texas/37/2024-neutralizing IgG Mapping of H5 serum neutralization among 76 historical and recent influenza A strains Isolation of H5/H1 cross-neutralizing monoclonal antibodies targeting the HA stem H5-neutralizing monoclonals protect mice from lethal H5N1 challenge  in vivo Summary The transmission of influenza A virus H5N1 clade 2.3.4.4b from cattle to humans highlights the risk of an H5N1 pandemic. Pre-existing immu

Highlights The lipoprotein-specific signal peptidase LspA is essential in  Pseudomonas aeruginosa LspA depletion compromises membrane integrity and increases antibiotic susceptibility A gain-of-function mutation in  amgS  supports growth under LspA-limiting conditions The membrane proteins HtpX and YccA are required for AmgRS-mediated rescue ABSTRACT Bacterial lipoproteins play crucial roles in cell envelope biogenesis, signaling, transport, and virulence, making the enzymes

The oral microbiome is a complex community of bacteria, fungi, and viruses that inhabit the oral cavity. Microbes of the oral microbiome are implicated in health and disease. We collected 220 unstimulated saliva samples from patients with periodontal disease and varying degrees of dental caries, as well as from subjects with no signs of oral disease. Metagenomic analysis of saliva revealed significantly higher abundance of periodontal pathogens in people with gum disease, and

Parabacteroides goldsteinii  is a Gram-negative, obligately anaerobic bacterium belonging to the genus Parabacteroides within the phylum Bacteroidota and family Tannerellaceae. It is a commensal microorganism naturally present in the human gut microbiota and has attracted increasing scientific interests due to its potential roles in metabolic regulation, immune modulation, and host–microbe interactions. Morphology and Characteristics P. goldsteinii  is a non-spore-forming, no

Highlights  Influenza virus infection significantly induces the expression of lncRNA GAS5 via type I/III interferons and IL-6 signal pathways. LncRNA GAS5 can encode a critical micropeptide GAS5-P50 which facilitates influenza virus replication. GAS5-p50 interacts with NOTUM to promote the activation of the Wnt/β-catenin pathway. ABSTRACT Long non-coding RNAs (lncRNAs) have been implicated in various cellular processes, including the regulation of gene expression and cellular

Highlights First global review linking polyphasic taxonomy to cyanobacterial metabolites in mangroves Catalogs cyanobacterial strains recognized only by modern morphological and molecular data Reveals high cyanobacterial diversity across mangrove ecosystem, including novel taxa Highlights diverse bioactivities: antimicrobial, anticancer, toxins, bioplastic Bridges taxonomy with biotech applications in drugs, agrochemicals, dyes, biofuels Abstract Extensive exploration of less

Highlights Sexual abstinence activates the hypothalamic-pituitary-adrenal (HPA) axis Sexual abstinence promotes tumor progression by inducing pregnenolone production Pregnenolone facilitates the formation of H3K9me3 to suppress ERV replication Pregnenolone antagonist is a highly promising candidate for anti-tumor therapy Summary Research into steroid hormones shaping tumor biology has gained increasing attention. Using multiple mouse tumor models, we show that pregnenolone pr

Highlights The gut metagenome is correlated with a host’s exhaled breath volatilome We devise a method to capture mouse breath using a murine ventilator Gnotobiotic mouse models help identify microbial contributions to exhaled breath The breath volatilome can signal the presence of the asthma-associated species  E. sireaum Summary The gut microbiota is crucial to health, yet implementation of microbiota-based therapeutics is limited by the lack of rapid diagnostics. We hypoth

Highlights Development of mouse model that closely mimics Haemophilus influenzae-mediated COPD exacerbation. Gene targeting approach reveals that cytosolic phospholipase 2-a (cPLA2-a) alters lung inflammatory response using COPD exacerbation experimental model. Also, cPLA2-a contributes to airway epithelium thickening in this clinical manifestation. Novel mechanism of bacterial infection-mediated COPD exacerbation involving cPLA2a. ABSTRACT Among the characteristics of chroni

The  history of dental treatments  dates back to thousands of years. The scope of this article is limited to the pre-1981  history . The earliest known example of  dental caries  manipulation is found in a  Paleolithic  man, dated between 14,160 and 13,820  BP . The earliest known use of a  filling  after removal of decayed or infected  pulp  is found in a Paleolithic who lived near modern-day  Tuscany , Italy, from 13,000 to 12,740 BP. Although inconclusive, researchers have

Highlights BPR2-D2, a coumarin derivative, is able to inhibit Chikungunya virus (CHIKV) RNA replication with a high selective index. BPR2-D2 can inhibit replication of Sindbis virus, which is an arthritogenic alphavirus as CHIKV The CHIKV replicon systems, which can be surrogates for studying CHIKV RNA replication, are applicable and convenient for antiviral screening. ABSTRACT Chikungunya virus (CHIKV), an alphavirus in the  Togaviridae  family, is transmitted to humans thro

Highlights Alternative splicing has been implicated in the regulation of a variety of genes Intron retention is the most frequent alternative splicing event in fungi We critically identified the gaps in the role of alternative splicing in fungi We proposed a practical roadmap to fill these gaps. ABSTRACT Assigning mechanistic and functional associations to most of the widespread splicing diversity in fungi has been one of the continuous and most intriguing challenges in the f

Porphyromonas gingivalis  is a keystone pathogen in periodontitis, known for its ability to invade gingival epithelial cells and persist intracellularly. Conventional antimicrobials are often ineffective against intracellular pathogens, and natural products remain poorly explored in this context. Here, we investigated the antimicrobial effects of  Boswellia serrata  extract and its bioactive compounds on the dynamics of  P. gingivalis  infection   in human gingival epithelial