Global Single‑Cell Genome Atlas Reveals Hidden Diversity and Metabolic Potential of Deep‑Ocean Prokaryoplankton

GORG‑Dark, a dataset of 9,698 single‑cell genomes from deep‑ocean prokaryoplankton, reveals extensive vertical stratification, unexpected particle‑attached Pelagibacterales, and high Patescibacteria and Nanoarchaeota abundance in marginal seas. Coding analyses uncover chemolithoautotrophy, novel proteorhodopsins, and secondary metabolisms across aphotic lineages.

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