Cryptic Genomic Heterogeneity in a Multidrug-Resistant Pseudomonas aeruginosa Left Ventricular Assist Device Isolate Revealed by Ultra-Deep Long-Read Sequencing

Using ultra-deep long-read sequencing, researchers characterized genomic heterogeneity in a multidrug-resistant Pseudomonas aeruginosa strain recovered from a left ventricular assist device infection. Rare structural variants occurring at frequencies as low as 0.0122% were detected, revealing previously inaccessible subpopulations and generating new hypotheses regarding biofilm formation, adaptation, antimicrobial resistance, and interactions with phage therapy.

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